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KMID : 0613820080180020206
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Journal of Life Science
2008 Volume.18 No. 2 p.206 ~ p.212
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Detection of a Quorum-Sensing Inhibitor from the Natural Products
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Kim Tae-Woo
Cha Ji-Young
Lee Jun-Seung
Min Bok-Kee
Paik Hyung-Suk
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Abstract
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The quorum sensing (QS) regulatory network has been the subject of extensive studies during recent years and has also attracted a lot of attention because it both positively and negatively regulates various putative virulence factors, although initially considered to be a specialized system of Vibrio fischeri and related species. In this study, to identify the novel materials which inhibit QS system of microorganisms, extracts of eighteen natural products were tested by bioassay using N-(3-oxohexanoyl)--homoserine lactone and N-(3-oxooctanoyl)--homoserine lactone synthesized in this experiment and an Agrobacterium tumefaciens NT1 biosensor strain containing a traI::lacZ fusion. The result indicated that the extracts of cabbage, leek, and onion exhibited the QS inhibition activity. Thus, materials contained in the extracts were isolated via recycling preparative HPLC and were purified via a JAIGEL-LS255 column. The common fraction corresponding to a peak of the 83 min point of them quenched the quorum sensing of A. tumefaciens NT1 biosensor strain in ABMM containing X-gal and was designated quorum sensing inhibitor-83 min (QSI-83). The QSI-83 exhibited the heat stability and did not inhibit the growth of A. tumefaciens NTl. Furthermore, thin layer chromatography (TLC) results suggested that these novel materials may be antagonists of N-acyl homoserine lactone or may inhibit the QS autoinducer synthesis by Pseudomonas syringae pv. tabaci.
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KEYWORD
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Quorum sensing (QS), N-acyl homoserine lactone (AHL), Pseudomonas syringae pv. tabaci, thin layer chromatography (TLC), recycling preparative HPLC
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